Journal: Frontiers in Pharmacology

Article Title: Optimizing the enzymatic release of MMAE from iso DGR-based small molecule drug conjugate by incorporation of a GPLG-PABC enzymatically cleavable linker

doi: 10.3389/fphar.2023.1215694

Figure Lengend Snippet: Flow cytometry analysis of integrin α V β 3 receptor level of expression in the four cancer cell lines used in this work.

Article Snippet: Afterwards, integrin α V β 3 antibody (Anti-Integrin α V β 3 antibody, clone LM609, mouse; MAB 1976, Merck) was used in a concentration of 3 µg/million cells, diluted in PBS and 3% BSA solution and incubated for 2 h at rt.

Techniques: Flow Cytometry, Expressing

Journal: Frontiers in Pharmacology

Article Title: Optimizing the enzymatic release of MMAE from iso DGR-based small molecule drug conjugate by incorporation of a GPLG-PABC enzymatically cleavable linker

doi: 10.3389/fphar.2023.1215694

Figure Lengend Snippet: Cell viability curves and calculated IC 50 values of cancer cell lines with different integrin α V β 3 receptor level of expression after 72 h of continuous treatment with the conjugate 2 , free drug MMAE, and free ligand. (A) U87MG. (B) SK-MEL-28. (C) SK-OV-3. (D) A549.

Article Snippet: Afterwards, integrin α V β 3 antibody (Anti-Integrin α V β 3 antibody, clone LM609, mouse; MAB 1976, Merck) was used in a concentration of 3 µg/million cells, diluted in PBS and 3% BSA solution and incubated for 2 h at rt.

Techniques: Expressing

Journal: Frontiers in Pharmacology

Article Title: Optimizing the enzymatic release of MMAE from iso DGR-based small molecule drug conjugate by incorporation of a GPLG-PABC enzymatically cleavable linker

doi: 10.3389/fphar.2023.1215694

Figure Lengend Snippet: Cell viability curves and calculated IC 50 values obtained after 30 min treatment of cancer cell lines with different integrin α V β 3 receptor level of expression with the conjugate 2 , free drug MMAE, and free ligand, followed by washout and further incubation of the cells up to 72 h (A) U87MG. (B) SK-MEL-28. (C) SK-OV-3. (D) A549.

Article Snippet: Afterwards, integrin α V β 3 antibody (Anti-Integrin α V β 3 antibody, clone LM609, mouse; MAB 1976, Merck) was used in a concentration of 3 µg/million cells, diluted in PBS and 3% BSA solution and incubated for 2 h at rt.

Techniques: Expressing, Incubation

Journal: Biomedicines

Article Title: Triazole Modified Tetraiodothyroacetic Acid Conjugated to Polyethylene Glycol, a Thyrointegrin α v β 3 Antagonist as a Radio- and Chemo-Sensitizer in Pancreatic Cancer

doi: 10.3390/biomedicines10040795

Figure Lengend Snippet: Signaling pathways significantly affected by the P-bi-TAT treatment of human pancreatic carcinoma SUIT2 cells.

Article Snippet: Vibrant MTT cell proliferation assay kit was purchased from Life Technologies Corporation (Carlsbad, CA, USA), and fluorescein isothiocyanate (FITC) conjugated antibody of integrin α v β 3 was purchased from BD Biosciences (San Jose, CA, USA).

Techniques: Ligand Binding Assay, Activity Assay, Homologous Recombination